Decolorization and degradation of Blue Hegn and Black B dyes by Bacillus Cereus isolated from the textile dye contaminated soil

Abstract

The soil samples were collected from Jamaratextile Industry,Perundurai, Erode district. The collected samples were subjected to serial dilution and plating technique. The isolated microbial strains were identified based on the morphology, biochemical test and 16s rRNA Sequence. The soil isolates were subjected to textile dye Blue HEGN and Black B degradation based on the decolorizing potential. The most efficient strain was chosen after the immobilization studies. The 16S rRNA sequencing of the most efficient bacterial strain RR4 showed 99% similarity to Bacillus cereus. The analysis of degradation products by FTIR and UV- Vis analysis revealed disruption of dyes linkages and subsequent conversion of aromatic amines into simpler products. Bioremediation has proved to be very effective method in countering the textile dye pollution in an eco-friendly way. This approach creates a promising hope to remediate the environments polluted by textile azo dyes.