In vitro rapid multiplication of Coldenia procumbens L. from shoot tip explants
DOI:
https://doi.org/10.46947/joaasr13201615Abstract
An efficient protocol for in vitro rapid multiplication of Coldenia procumbens L. has been developed. The shoot tip explants were cultured on MS basal medium supplemented with different concentrations of BAP and KIN. The two cytokinins are tested, BAP was found to develop higher number of shoots from the shoot tip explants when compared to KIN. higher number of shoots was produced from all the concentrations of BAP and KIN. The highest frequency of 100% shoot induction was observed on MS basal medium supplemented with 6 μM BAP and 10 μM KIN. The number of shoots observed on the MS basal medium supplemented with BAP obtained 14 as well as 11 in KIN. The data's are recorded after 30days of inoculation.
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