In vitro Shoot multiplication of Physalis minima L. - an important Medicinal Herb

Abstract

The present study was to develop an efficient protocol for shoot multiplication of Physalis minima L. Shoots were cultured using nodal segments from 20 days old field grown young plant. The nodal explants were cultured on MS medium supplemented with different concentrations of BAP and KIN. The two cytokinins tested, KIN was found to respond well in shoot multiplication and number of shoots from the nodal explants when compared to BAP. Large number of shoots was induced from all the concentrations of both BAP and KIN. BAP was found to develop in shoot multiplication and higher number of shoots from the nodal explants when compared to KIN. The highest frequency of 100% shoot induction was observed on MS basal medium supplemented with 8μM BAP and 10 μM KIN. Matured shoots are isolated and then transferred to the MS basal medium supplemented with  different concentration of NAA and IBA for root induction. The higher number of roots were produced in 30 days on MS basal medium supplemented with 10 μM IBA and 6 μM NAA. The well rooted plantlets were isolated and transplanted to the paper cup for hardening and the well established plants were transferred to the field for acclimatization.