Assessment of the Radical Scavenging Activity and Total phenolic content of Crude Methanolic leaves Extract of Aristolochia indica L.- Aristolochiaceae

Abstract

The present study was aimed to screen antioxidant and quantify the phytoconstituents of Aristolochia indica leaves extracts evaluated the antioxidant in three methods viz. DPPH free radical scavenging activity, phosphomolybdenum method and reducing power assay. The total phenols and total flavonoids were quantitatively estimated in leaves of A. indica. The results thus obtained were compared with standard antioxidant compound like ascorbic acid. The results revealed that free radical scavenging activity of methanolic leaf extract of A. indica has radical scavenging ability on DPPH with IC₅₀ value of 38.72µg/ml. The positive control ascorbic acid showed the IC₅₀ values of 32.42µg/ml. The total phenols and flavonoids content in leaf extract indicated the antioxidant activity. The present study provides evidence that solvent extract of A. indica contain important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.