Extraction and Identification of β-carotene from unripe date palm

Gayathri Maheswaran, Dr.S. Thilagavathi

Abstract


Carotenoids are one of the natural plant pigment and also valuable preventive medicines too. Recent research shows that there is solid connection between the eating regimen wealthy in carotenoids with expanded assurance against cardiovascular sickness and particular sorts of malignant growth. The primary target of the current investigation is to remove the carotenoids from two varieties of unripe Phoenix dactylifera namely Barhi (yellow in colour, YD) and Khenaizi (red in colour, RD) and then to identify the presence of β-carotene by using preparative “Thin layer choromatography” (TLC) and “High Performance Liquid Chromatographic” (HPLC) technique. The experimental results of both the samples were compared to standard β-carotene. From the results obtained both the samples were contain notable amount of β-carotene, Mainly Khenaizi shows more retention time (5.09) compared to that of Barhi (5.02).β-carotene is useful in giving likely advantage to forestalling nutrient vitamin A lack and it is perhaps the most common public health problems.

Keywords


Phoenix dactylifera, β-carotene, Thin layer chromatography, High performance liquid chromatography, vitamin A deficiency.

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References


REFERENCES

Al‐Shahib W, Marshall RJ. Fatty acid content of the seeds from 14 varieties of date palm Phoenix dactylifera L. International journal of food science & technology. 2003 Aug;38(6):709-12

Bendich A. From 1989 to 2001: what have we learned about the “biological actions of beta-carotene”?. The Journal of nutrition. 2004 Jan 1;134(1):225S-30S.

Butnariu M. Methods of analysis (extraction, separation, identification and quantification) of carotenoids from natural products. J. Ecosyst. Ecography. 2016 Jun;6(2):1-9.

Das S, Gupta P, De B. Thin Layer Chromatographic Characterization of Carotenoid Isolates in Sugar Date Palm (Phoenix sylvestris) Fruit Epicarp and Inflorescence Axis. Internat. J. Pharmacognosy& Phytochemical Res. 2017;9(5):680-4.

De Sá MC, Rodriguez-Amaya DB. Carotenoid composition of cooked green vegetables from restaurants. Food Chemistry. 2003 Dec 1;83(4):595-600.

Kiokias S, Dimakou C, Oreopoulou V. Activity of natural carotenoid preparations against the autoxidative deterioration of sunflower oil-in-water emulsions. Food chemistry. 2009 Jun 15;114(4):1278-84.

Lakshminarayana R, Raju M, Krishnakantha TP, Baskaran V. Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry. 2005 Apr 20;53(8):2838-42.

Merculieff Z, Ramnath S, Sankoli SM, Venkataramegowda S, Murthy GS, Ceballos RM. Phytochemical, antioxidant and antibacterial potential of Elaeagnuskologa (Schlecht.) leaf. Asian Pacific journal of tropical medicine. 2014 Sep 1;7:S599-602.

Paiva SA, Russell RM. β-carotene and other carotenoids as antioxidants. Journal of the American college of nutrition. 1999 Oct 1;18(5):426-33.

Rodriguez-Amaya DB. Status of carotenoid analytical methods and in vitro assays for the assessment of food quality and health effects. Current Opinion in Food Science. 2015 Feb 1;1:56-63.

Saleh EA, Tawfik MS, Abu-Tarboush HM. Phenolic contents and antioxidant activity of various date palm (Phoenix dactylifera L.) fruits from Saudi Arabia. Food and Nutrition Sciences. 2011 Dec 19;2011.

Smithagrace,S.R, JyotiBala Chauhan and ChitraRatnakar Jain, “Preliminary phytochemical investigation and TLC analysis of peel and seed extracts of Persea American”, Asian Journal of Pharm Science and Tech, 2015, Vol(5), 167-171.

Soares LL, Bassani VL, Ortega GG, Petrovick PR. Total flavonoid determination for the quality control of aqueous extractives from Phyllanthusniruri L. ActaFarmaceuticaBonaerense. 2003;22(3):203-8.

Tomkins SP, Miller MB. A rapid extraction and fast separation of leaf pigments using thin layer chromatography. School science review. 1994;75:69.

Varzakas T, Kiokias S. HPLC analysis and determination of carotenoid pigments in commercially available plant extracts. Current Research in Nutrition and Food Science Journal. 2016 Mar 25;4(Special Issue Carotenoids March 2016):01-14.

Veeresham C. Natural products derived from plants as a source of drugs. Journal of advanced pharmaceutical technology & research. 2012 Oct 1;3(4):200.

Yamaguchi, M and Uchiyama, S., “Effect of Carotenoids on Calcium content and alkaline phosphatase activity in rat femoral tissues in-vitro: the unique anabolic effect of beta cryptoxanthin,” 2003, 26(8), 1188-1191.


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